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Conventional mineral salts medium (MSM) had been used for many years. A modification of the concentration of sodium chloride was attempted in this work to observe the effect or otherwise on microbial activities in reaction tubes. This study was undertaken to assess the biodegradation potentials of Alcaligenes species on heptane.
Results showed the hydrocarbon degrading ability of Alcaligenes sp. under varying concentrations of sodium chloride salt for a period of sixteen (16) days at two to four days interval. Due to its rapid rate of multiplication, a steady increase in bacterial growth was observed during the experiment. Alcaligenes sp. showed appreciable growth on heptane with a reading of 6.5x108cfu/ml on Day 16. Also, the regeneration rate of Alcaligenes sp. was found to be rapid on heptane in the presence of 4M concentration of NaCl with a reading of 1.18x109cfu/ml on Day 16. This study shows that increased sodium chloride concentration aids the utilization of heptane by Alcaligenes sp. Hence, Alcaligenes sp. is a promising isolate that can be used for the bioremediation of hydrocarbon contaminated sites in saline environment.
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