An Ethnobotanical Study of Herbal Contraceptives Used by Local Medicine Practitioners in Bida, Niger State, Nigeria
Main Article Content
Abstract
Background/Aim: Most plants are naturally endowed with curative abilities now commonly sourced in phytotherapy. Medicinal plants usage for regulating fertility in Nigeria is popular due to their effectiveness, cheapness, easy accessibility and minimal side effects. Nigeria is the most populous country in Africa with about 218.5 million people as at 2021 and 232.68 - 237.5 million in late 2024 to 2025 mid-year; where birth rate outnumbers the death rate and most users are young women who prefer the medicaments after sexual intercourse; so, there is the need to control birth rate to prevent further escalation in population growth. Methods: Questionnaires were administered to both the Traditional Medicine Practitioners (TMPs) and users of herbal birth control medicines in three major markets in Bida metropolis, Niger state. Results: Data presented are from the ethnobotanical survey from 220 participants. The names of the plants used as contraceptives or abortifacients and the methods of preparation of these recipes are presented. The results from the survey contained 26 species of medicinal plants including Crotalaria mucronata, Mangifera indica, Securidaca longepedunculata and Vernonia amygdalina. The most frequently used plants for contraception or abortifacient are Newbouldia laevis, Ricinus communis and Azadirachta indica. Conclusion: Further pharmacological screening into the reported plants distributed in 28 genera belonging to 24 families is strongly suggested to ascertain or disprove the claims of the TMPs.
Downloads
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors hold the copyright of all published articles except otherwise stated.
References
Abubakar, A. R. and Haque, M. (2023). Advancing herbal medicine: enhancing product quality and safety through contemporary techniques. Frontiers in analytical Pharmacology, 14: 1265178.
Adebayo, B., Olorunfemi, O. and Moronkola, O. (2013). An examination of the usage of herbal contraceptives and abortifacients in Lagos State, Nigeria. Journal of African Studies and Development, 5(5): 114–122.
Ademoye, M. A., Lajide, L., Owolabi, B. J. and Onubogu, C. C. (2018). GCMS analysis of raw and roasted seeds of Chrysophyllum albidum, a medicinal plant used for the treatment of tuberculosis. International Journal of Scientific Management, 6(08): 18–27.
Adeniyi, A. A. and Alo, O. (2025). Exploring the Male Antifertility Potential of Medicinal Plants. Pharmacognosy Research, 17(2), 462-475.
Adeyemi, M. M. H. and Idris, M. A. (2022). Reproductive Effects of Medicinal Plant (Azadirachta indica) Used as Ethnoveterinary Medicine in Breeding Female Rats. Molecules, 27(16): 5174.
Adia, M. M., Kamatenesi-Mugisha, M., Musaya, S. and Rupwom, J. G. (2025). Medicinal plant species used for contraception and reproductive health care in rural communities of Western Uganda. Heliyon, 11(1): e000000.
Ahmed, M. E., Senabe, J., Yahaya, E. S., Fouche, G., Steenkamp, P. and Steenkamp, V. (2022). Isolation and antioxidant activity of 5 methyldihydroflavasperone from ethanol leaf extract of Guiera senegalensis JF Gmel. Journal of Medicinal Plants for Economic Development, 6(1): 137.
Ahmed, M. U., Arise, R. O., and Umaru, I. J. (2022). Identification and biochemical characterization of anti-enteropooling compounds from Annona senegalensis root bark. Scientific African, 15: e01128.
Aina, O. O. and Aina-Pelemo, A. R. (2019). Production and Development of Herbal Contraceptives in Nigeria: The Roles of the Ethnobotanist and Standards Organization of Nigeria. Global Journal of Research in Engineering, 19(2): 35–42.
Aladejare, T. T., Obuotor, E. M. and Aderogba, M. A. (2018). Isolation and characterization of antioxidant compounds from Annona senegalensis leaf extracts. Nigerian Journal of Natural Products and Medicine, 22: 124-128.
Alahmadi, B. A. (2020). Effect of Herbal Medicine on Fertility Potential in Experimental Animals – an Update Review. Materia Socio-Medica (Mater Sociomed), 32(2): 140-147.
Al-Taweel, A. M., Perveen, S., Fawzy, G. A., Rehman, A. U., Khan, A., Mehmood, R. and Fadda, L. M. (2017). Evaluation of antiulcer and cytotoxic potential of the leaf, flower, and fruit extracts of Calotropis procera and isolation of a new lignan glycoside. Evidence-Based Complementary and Alternative Medicine, 2017: 1-14.
Ambali, O. A., Oguntimehin, S. A., Ajaiyeoba, E. O., Ogbole, O. and Adeniji, A. J. (2021). Bioassay-guided isolation of cytotoxic constituents of Aframomum melegueta K. Schum. seeds. Nigerian Journal of Natural Products and Medicine, 25(1): 80-85.International Journal of Scientific Reports, 10(1): 1384.
Dirar, A. I., Adhikari-Devkota, A., Hassan, M. M., Wada, M., Watanabe, T. and Devkota, H. P. (2019). Phenolic compounds as potent free radical scavenging and enzyme inhibitory components from the leaves of Guiera senegalensis. Natural Product Communications, 14(6): 1934578X19857364.
Djeujo, F. M., Stablum, V., Pangrazzi, E., Ragazzi, E. and Froldi, G. (2023). Luteolin and vernodalol as bioactive compounds of leaf and root Vernonia amygdalina extracts: effects on α-glucosidase, glycation, ROS, cell viability, and in silico ADMET parameters. Pharmaceutics, 15(5): 1541.
Dugoua, J. J., Perri, D., Seely, D. and Koren, G. (2008). Safety and efficacy of blue cohosh (Caulophyllum thalictroides) during pregnancy and lactation. Canadian Journal Pharmacology, 15(1): e66-e73. of Clinical Dzoyem,
J. P., Nganteng, D. N. D., Melong, R., Wafo, P., Ngadjui, B., Allémann, E. and Delie, F. (2021). Bioguided triterpenoids constituents identification as of anti-inflammatory of Ocimum pentacyclic bioactive gratissimum extract. Journal 113637. of Ethnopharmacology, 268:
Ebbo, A. I., Bello, A., Ismail, M. S., Dauda, A. M., Idris, S. B. and Sani, A. A. (2019). Ethno-Veterinary Survey of Medicinal Plants used for the Treatment of Ruminant Ailments in Billiri Local Government Area of Gombe State, Nigeria. Direct Research Journal of Health and Pharmacology, 7(3): 42-51.
Egga, S. E., Ekwenchi, M. M., Theophilus, A., Achuenu, C. and Lokta, S. D. (2023). GC-MS analysis and the establishment of mass spectrometric fingerprints of chemical constituents present in ethanol extract of Securidaca longipedunculata leaves. World Journal of Pharmaceutical Research, 12(4): 2152–2167.
Ellington, S. R., Kourtis, A. P., Curtis, K. M. et al. (2013). Contraceptive availability during an emergency response in the United States. Journal of Women’s Health (Larchmt), 22: 189–93.
Elmi, A., Spina, R., Risler, A., Philippot, S., Mérito, A., Duval, R. E., ... and Laurain-Mattar, D. (2020). Evaluation of antioxidant and antibacterial activities, cytotoxicity of Acacia seyal Del bark extracts and isolated compounds. Molecules, 25(10): 2392.
Fadzil, S. R., Yap, A. C., and Choo, Y. M. (2017). A New cyclopentylidene and other chemical constituents from Malaysian Crotalaria Malaysiana, 46(9): 1581-1586. pallida. Sains
Joel, O. J., Bamidele, J., Joel, A. F. and Sennuga, S. O. (2025). The Role of Traditional Medicine in Public Health Systems: Perspectives from Practitioners, Healthcare Professionals and Patients in Abuja, Nigeria. International Journal of Clinical Case Reports and Reviews, 25(3); DOI:10.31579/2690 4861/768
John, E. O., Oloye, T. S., Ekere, K., Ajeh, J. I., Abdullahi, R., Olayemi, O. et al. (2023). Knowledge, attitude and perception of contraceptive: Nigerians towards herbal A questionnaire – based study. Journal of Complementary and Alternative Medical Research, 23(4): 33–46.
Kamal, R., Gupta, R. S. and Lohiya, N. K. (2003). Plants for male fertility regulation. Phytotherapy Research, 17(6): 579–90.
Kamble, S. and Patil, S. (2025). Emerging trends in advanced herbal pharmaceuticals. GSC Biological and Pharmaceutical Sciences, 30(01): 221-229.
Kanwal, Q., Hussain, I., Siddiqui, L. H. and Javaid, A. (2011). Antimicrobial activity screening of isolated flavonoids from Azadirachta indica leaves. Journal of the Serbian Chemical Society, 76(3): 375-384.
Kaunitz A. M. (1992). Oral contraceptives and gynecologic cancer: an update for the 1990s. American Journal of Obstetrics and Gynecology, 167(4 Pt 2): 1171-6. doi: 10.1016/s0002-9378(12)90407-x. 1415442. PMID:
Kauntz, A. M. (2016). Contraceptive counselling and selection. Up to Http/www.update.com/contents/contraceptive. Counselling-and-selection. date. Kayode, J. (2002). Conservation and Ethnobotanical Exploration of Compositae in Ekiti State, Nigeria. Compositae Newsletter, 34: 62-68.
Krishnasamy, V. and Muruhan, S. (2021). Evaluation of Preliminary Phytochemical Screening, Antioxidant Activity, Isolation Bioactive and Characterization of Compounds in Waltheria indica Linn. Journal of Pharmaceutical International, 33(62A): 129-143.
Liu, G. Z. and Lyle, K. C. (1987). Clinical trial of gossypol as a male contraceptive drug. Part II. Hypokalaemia study. Fertility and Sterility, 48(3): 462–465.
Lohiya, N. K., Manivannan, B., Mishra, P. K. and Pathak, N. (2001). Prospects of developing a plant based male contraceptive pill. In: Chowdhury, S. R., Gupta, C. M., Kamboj, V. P. editors. Current Status in Fertility Regulation: Indigenous and Modern Approaches. Lucknow: Central Drug Research Institute, pp 99-119 Infertility in Ondo State, Nigeria. Pakistan Journal of Life and Social Sciences, 23(1): 9212 – 9235.
Okoye, T. C., Akah, P. A., Omeje, E. O., Okoye, F. B., & Nworu, C. S. (2013). Anticonvulsant effect of kaurenoic acid isolated from the root bark of Annona senegalensis. Pharmacology Biochemistry and Behavior, 109, 38-43
.Olatunji-Bello, I., Aliu, O. N. (2000). On the anti-fertility effects of castor seeds (Ricinus communis). Journal of Medicine and Medical Sciences, 2(1): 74 - 76.
Oluwasegun, A., Ogochukwu, U., Ugochukwu, O., Mussaddiq, I. and Bunyamin, A. (2023). Lupeol: A Triterpenoid Isolated from the Stem Bark of Hymenocardia acida (Tul.) Exhibits a van der Waal Antagonism on the Alpha Subunit of Gastric H+ K+ Atpase–A Promising Antiulcer Principle. Drug Research, 73(08): 448-458.
Oraibi, A. I. and Hamad, M. N. (2018). Phytochemical investigation of flavanoid of Calotropis procera in Iraq, Isolation and identification of rutin, quercitin and kampferol. Journal of Pharmaceutical Sciences and Research, 10(9): 2407-2411.
Pan, J., Yi, X., Zhang, S., Cheng, J., Wang, Y., Liu, C., and He, X. (2018). Bioactive phenolics from mango leaves (Mangifera indica L.). Industrial Crops and Products, 111: 400-406. Parcha,
V., Dobhal, Y., and Dhasmana, D. C. (2019). Isolation, characterization and cardioprotective potential of gratissinol from chloroform extract of Ocimum gratissimum (Linn.) Leaves. Journal of Biologically Active Products from Nature, 9(4): 260-268.
Piccinelli, A. L., García Mesa, M., Armenteros, D. M., Alfonso, M. A., Arevalo, A. C., Campone, L. and Rastrelli, L. (2008). HPLC-PDA-MS and NMR characterization of C-glycosyl flavones in a hydroalcoholic extract of Citrus aurantifolia leaves with antiplatelet activity. Journal of Agricultural and Food Chemistry, 56(5): 1574-1581. Population Reference Bureau (PRB). (2016). World Population Data Sheet 2 Pp. 20.Population Reference Bureau (PRB). (2024). 2024 World Population Data Sheet. https://2024-wpds.prb.org/
Praveena, K. and Gajalakshmi, G. (2025). A Comprehensive Review on Neem Seed Oil as a Natural Contraceptive. International Journal Pharmaceutical Research, 17(1). Sciences Review
Priya, A., Singh, B. and Devi, P. (2025). A Comprehensive Review on Neem Seed Oil as a Natural Contraceptive. International Journal Pharmaceutical Sciences, 16(1): 71-80. Of preclinical studies. Journal of pharmaceutical sciences, 109(1): 475-485.
Udoh, P., Kehinde, A. (1999). Studies on anti-fertility effect of pawpaw seeds (Carica papaya) on the gonads of male albino rats. Phytotherapy Research, 13: 226 228. Ugbogu, E. A., Emmanuel, O., Dike, E. D., Agi, G. O., Ugbogu, O. C., Ibe, C. Iweala, E. J. (2021). The phytochemistry, ethnobotanical, and pharmacological potentials of the medicinal plant Vernonia amygdalina L. (bitter Leaf). Clinical Complementary Medicine and Pharmacology, 1(1), 100006. Unegbu, C. C., Uzohuo, E. N., Ajah, O., Anyanwu, O. O. and Anyanwa, C. (2021). Extraction and characterization of alkaloid and tannin from Newbouldia laevis leaves. Vildina, J. D., Ndjonka, D., Schmidt, T. J. and Liebau, E. (2021). Identification of anti-
Caenorhabditis and anti-Onchocerca constituents from leaves of Annona senegalensis Pers. (Annonaceae). South African Journal of Botany, 138: 84-93.
Wanzala, W., Zessin, K. H., Kyule, M. N., Baumann, M. P. O., Mathias, E., and Hassan, B. (2005). Ethnoveterinary practices in the pastoral Maasai communities of Kajiado District, Kenya. Tropical Animal Production, 37(S1), 23-33.
Health and Amir-Zargar, H., Ghorbani, A. and Shahrestani, S. (2022). The most important medicinal plants affecting sperm and testosterone production: a systematic review. Journal of Brazilian Association of Assisted Reproduction (JBRA Ass Reprod), 26(3): 522-530.
Bader, A., Abdelhady, M. I., Shaheen, U., El-Malah, Y., Abourehab, M. A. S. and Barghash, M. F. (2015). Azadirachta indica as a source for antioxidant and cytotoxic polyphenolic compounds. Biosciences Biotechnology Research Asia, 12(2): 1209-1222.
Banakar, P. and Jayaraj, M. (2018). GC-MS analysis of bioactive compounds from ethanolic leaf extract of Waltheria indica Linn. and their pharmacological activities. International Journal of Pharmaceutical Sciences and Research, 9(5): 2005-2010.
Banerjee, D., De, B., Bhowmick, S., Roy, D. and Reang, D. (2025). Seeds of Plants as Natural Contraceptives. Acta Scientific Pharmaceutical Sciences, 9(8): 03-12. Birhan, Y.S. (2022). Medicinal plants utilized in the management of epilepsy in Ethiopia: ethnobotany, pharmacology and Medical Journal phytochemistry. Chinese 17: 129 https://doi.org/10.1186/s13020-022-00686-5
Chandramouly, A., Ganesan, N. and Venkatesan, P. (2024). Utilisation of medicinal plants for their antifertility activities. Heliyon, 10(15), e000551. doi.org
Chauhan, A., Agarwal, M., Kushwaha, S. and Mutreja, A. (2007). Suppression of fertility in male albino rats following the administration of 50% ethanolic extract of Aegle marmelos Contraception, 76: 474 481.
Chen, H., Fu, J., Chen, H., Hu, Y., Soroka, D. N., Prigge, J. R., Schmidt, E. E., Yan, F., Major, M. B., Chen, X. and Shen, T. L. (2014). Ginger compound [6] shogaol and its cysteine-conjugated metabolite (M2) activate Nrf2 in colon epithelial cells in vitro and in vivo. Chemical Toxicology, 27(9): 1575–1585.
Research in Chen, H., Xu, Y., Chen, H., Liu, H., Yu, Q. and Han, L. (2022). Isolation and identification of polyphenols from fresh sweet sorghum stems and their antibacterial mechanism against foodborne pathogens. Frontiers in Bioengineering and Biotechnology, 9: 770726.
Ciganda, C. and Laborde, A. (2003). Herbal Infusions used for induced abortion. Journal of toxicology and Clinical toxicology, 41(3): 235–239. David, D., Airaodion, A. I. and Ekenjoku, J. A. (2024). Anti implantation and uterotonic properties of Mentha pulegium L. in female Sprague-Dawley rats.
Fahmy, N. M., Al-Sayed, E. and Singab, A. N. (2015). Genus Terminalia: A phytochemical and biological review. Medicinal and Aromatic Plants, 4(5): 1-22.
Farnsworth, N. P. (1975). Potential value of plants as sources of new antifertility agents. Part 1. Journal of Pharmaceutical Sciences, 64 (April): 535-598. Part 2 64 (May): 717-754.
Feng, C., Fay, K. E., Burns, M. M. (2023). Toxicities of herbal abortifacients. American Journal of Emergency Medicine, 68: 42-46. doi: 10.1016/j.ajem.2023.03.005. PMID: 36924751; PMCID: PMC10192026. Goswami, P. and Basak, M. (2021). Medicinal Plants Exhibiting Antifertility Activity: A Review. Journal of Pharmaceutical Research International, 33(60B): 230–237. doi.org.
Gupta, R. S., Kachhawa, J. B. and Chaudhaury, R. (2004). Antifertility effects of methanolic pod extract of Abizzia lebbeck. Asian Journal of Andrology, 6: 155-159. Hoet, S., Opperdoes, F., Brun, R., Adjakidje, V. and Quetinleclercq, J. (2004). In vitro anti trypanosomal activity of ethnopharmacologically selected Beninese plants. Journal of Ethnopharmacology, 91(1): 37-42.
Hubscher, G. H., Brook, D. L. and Johnson, J. A. (2005). A quantitative method for assessing stages of the rat estrous cycle. Biotechnic and Histochemistry, 80 (2): 79-87.
Hussein, A. O., Hameed, I. H., Jasim, H. and Kareem, M. A. (2015). Determination of alkaloid compounds of Ricinus communis by using gas chromatography mass spectroscopy (GC-MS). Journal of Medicinal Plants Research, 9(10): 349-359.
Iftikhar, S., Abbas, R., Zafar, U. and Fatima, S. (2022). Determination of possible contraceptive potential of methanolic leaf extract of Mentha longifolia L. on the reproductive system of male rats by assessing sperm parameters, histological, and biochemical analysis. PubMed Central (PMC): 9773058: 13740.
Indriyani, N. N., Anshori, J. A., Permadi, N., Nurjanah, S. and Julaeha, E. (2023). Bioactive Components and Their Activities from Different Parts of Citrus aurantifolia (Christm.) Swingle for Food Development. Foods, 12(10): 2036.
Jahan, S., Saeed, N., Ijla, l. F., Khan, M. A., Ahmad, M., Zafar, M. and Abbasi, A. M. (2009). Histomorphological study to evaluate anti-fertility effect of Abrus precatorius L. in adult male mice. Journal of Medicinal Plants Research 3(12): 1021- 1028.
Mbatchou, V. C., and Oumar, A. A. (2012). Antifungal activity of Nilobamate isolated from Acacia nilotica Wild. Phytopharmacology, 3(1), 208-213.
Muhammad, M. J., Mann, A., Fadipe, L. A., Kamdem, M. H. K., Mmutlane, E. M. and Ndinteh, D. T. (2023). Isolation of Stigmasterol and Gastroprotective Activity of the Ethyl Acetate Portion from Acacia nilotica Linn. (Fabaceae) Seeds. Journal of Chemical Society of Nigeria, 48(1): 12 - 21.
Nacira, B., Mebarka, B., Yacine, L., Mohamed, H., Roukia, H., and Mahfoud, H. M. (2023). Isolation and structural elucidation of phenolic components from Calotropis procera (ait) and evaluation of insecticidal activity. Journal of Animal and Plant Sciences, 33(4): 770-779.
Ndoye, S. F., Tine, Y., Seck, I., Ba, L. A., Ka, S., Ciss, I., ... and Seck, M. (2024). Chemical Constituents and Antimicrobial and Antioxidant Activities of Essential Oil from Dried Seeds of Xylopia aethiopica. Biochemistry Research International, 2024, 1-10, Article ID 5914321.
Nicolas, M. A., Alvine, N. M., Gaëtan, B. B. N., Kevin, M. D. Y., Darline, D., Alida, T. E. M., ... and Emmanuel, P. D. (2021). Bioguided isolation, structure elucidation and evaluation of new antimicrobial flavonoid from Carica papaya leaves. Journal of Pharmacognosy Phytochemistry, 10(1), 32-39.
Noumi, N. Y. C. and Tchakonang, C. (2001). Plants used as abortifacients. Ethnopharmacology, 76(3): 263 268. National Population Commission, 2015. Nowak, J., Kiss, A. K., Wambebe, C., Katuura, E. and Kuźma, Ł. (2022). Phytochemical analysis of polyphenols in leaf extract from Vernonia amygdalina Delile plant growing in Uganda. Applied Sciences, 12(2), 912.
Nugroho, A., Heryani, H., Choi, J. S. and Park, H. J. (2017). Identification and quantification of flavonoids in Carica papaya leaf and peroxynitrite-scavenging activity. Asian Pacific Journal of Tropical Biomedicine, 7(3), 208-213.
Ochora, D. O., Kakudidi, E., Namukobe, J., Heydenreich, M., Coghi, P., Yang, L. J., … and Yenesew, A. (2022). A new benzophenone, and the antiplasmodial activities of the constituents of Securidaca longipedunculata fresen (Polygalaceae). Natural Product Research, 36(11), 2758-2766
Ogunbodede, A. J. (1997). Mental illness and traditional therapy in Nigeria, Ibom Journal of Social Issues 4, pp. 56-66. Oguntibeju, O. O. and Owira, P. M. (2025). Selected Medicinal Plants Used in the Treatment of Male
Punia, J. and Singh, R. (2018). Isolation, characterization and biological potential of compounds from flowers of Moringa oleifera. Journal of Indian Chemical Society, 95: 1107-1120.
Qian, S. Z. (1987). Tripterygium wilfordii, a Chinese herb effective in male fertility regulation contraception. International Journal of Medicinal Plants Research, 36(3): 335–345.218. Salem,
M. M., Davidorf, F. H. and Abdel-Rahman, M. H. (2011). In vitro anti-uveal melanoma activity of phenolic compounds from the Egyptian medicinal plant Acacia nilotica. Fitoterapia, 82(8): 1279 1284.
Sathiyaraj, K., Sivaraj, A., Vinoth K. P., Devi, K., Senthil, K. B. (2010). Spermicidal Activity of Azadirachta indica (Neem) Aqueous Leaf Extract on Male Albino Rats. International Journal of Pharmacy Research and Technology, 2 (1): 588- 591.
Sethi, J., Singh, J., Gurung, N. and Aggarwal, A. (2019/2025). Medicinal Plants as a Potential Source of Male Contraceptive Agents. Madridge Journal of Pharmaceutical Research, 3(2):116-121.
Sewani-Rusike, C. R. and Nkomo, M. (2022). Phytochemical Profile, Safety and Efficacy of a Herbal Mixture Used for Contraception by Traditional Health Practitioners in Ngaka Modiri Molema District Municipality, South Africa. Molecules, 27(2): 193.
Shaik, A., Yalavarthi, P. R. and Bannoth, C. K. (2017). Role of Anti-Fertility Medicinal Plants on Male & Female Reproduction. Journal of Complementary and Alternative Medical Research, 3(2): 1–22. https://doi.org/10.9734/JOCAMR/2017/34632.
Shimbe, R.Y., Tor-Anyiin, T.A., Khan, M.E. & Anyam, J.V. (2016). β-sitosterol from Hymenocardia acida root extract and its antimicrobial activity. Journal of Chemical Society of Nigeria, 41(1). Singh A, Singh, SK. 2009. Evaluation of antifertility potential of Brahmi in male mouse. Contraception, 79:71–79.
Sofowora, E.A. (2006). Medicinal Plants and Traditional Medicine in Africa. 2nd Edn., Spectrum Books Limited, Ibadan, pp 151.
Tiwaskar, G. H., Mahure, P., Mowade, P., Tiwari, A., Sawarkar, K. and Pise, A. G. (2023). Exploring The Spermicidal Properties of Extracted Neem Seed Oil: An Ex-Vivo Study Investigation Based on Tribal Knowledge. European Chemical Bulletin, 12(10): 7793-7812. Top 10 Home Remedies Team, 2018. Four Herbs that act as Natural Birth http//www.top10homeremedies.com/wp contents/uploads. Control.
Tran, D. S., Antonyan, A. and Polli, J. (2020). Herbal product development for female fertility regulation: WHO (2000). General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine. Geneva, Switzerland. pp 1-80.
WHO (2003). Calls on African Governments to Formally Recognize Traditional Medicine. Johannesburg, South Africa. WHO (2023). Integrating Traditional Medicine in Health Care. World Health Organization, Regional Office for South-East Asia. 1.3.3 World Health Organization (WHO). WHO Medicines Strategy: 2000–2003: Framework for Action in Essential Drugs and Policy (WHO/EDM/2000.1). Geneva: Medicines World Health Organization; 2000. This document is available on the Reproductive Health Supplies Coalition website.
Yamasaki, R. B., Klocke, J. A., Lee, S. M., Stone, G. A. and Darlington, M. V. (1986). Isolation and purification of azadirachtin from neem (Azadirachta indica) seeds using flash chromatography and high performance liquid chromatography. Journal of Chromatography A, 356: 220-226. Zhao,
Y., Tao, Q. F., Zhang, R. P., Zhou, C. X., Dou, H., Shi, S. Y. and Li, X. K. (2007). Two new compounds from Zingiber officinale. Chinese Chemical Letters, 18(10): 1247-124